you can ask these of the initial screener but they probably wont have the answers. After they pass your resume on to the hiring manager and they call you there is no reason not to ask these questions. they will or should ask you if have any questions and that is the opportunity to ask. I think you will impress the interviewer with these questions and they in my experience they will answer as best as possible and their answers will allow you to honestly assess the opportunity for its appropriateness to you
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you can ask these of the initial screener but they probably wont have the answers. After they pass your resume on to the hiring manager and they call you there is no reason not to ask these questions. they will or should ask you if have any questions and that is the opportunity to ask. I think you will impress the interviewer with these questions and they in my experience they will answer as best as possible and their answers will allow you to honestly assess the opportunity for its appropriateness to you