If you are directing all of your subdomains to same IP address, you'll have to configure your web server to handle your subdomains differently. For example yourdomain.com, sub1.yourdomain.com and sub2.yourdomain.com will be directed to same IP address but yourdomain.com will run code at path /var/www/html, sub1 will run application at path /var/www/sub1, and sub2 will run code at /var/www/sub2.
It's not Route53, it's your web server that needs to be configured. If you're using Nginx, following article can help you. dev.to/on_stash/configure-nginx-to...
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If you are directing all of your subdomains to same IP address, you'll have to configure your web server to handle your subdomains differently. For example yourdomain.com, sub1.yourdomain.com and sub2.yourdomain.com will be directed to same IP address but yourdomain.com will run code at path /var/www/html, sub1 will run application at path /var/www/sub1, and sub2 will run code at /var/www/sub2.
It's not Route53, it's your web server that needs to be configured. If you're using Nginx, following article can help you.
dev.to/on_stash/configure-nginx-to...