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In this article for the first time, we have reported, a facile way for the creation of E.coli impressions in the polymer for selective capture and to destroy E. coli in drinking water. This microporous imprinted polymer has shown the existence of micrometer size rod shape cavities with the population of 2.45 × 102 ± 60 imprints per cm2. Adsorption capacity of the polymer for E.coli was 103 CFU mg-1. This microporous imprinted polymer captured 99% of the bacteria within 30 min at initial concentration of 109 CFU mL-1. The non-imprinted polymer prepared without the bacteria imprinting reported only 40% of the bacteria removal even after 60 min. The reduced graphene oxide was embedded in the microporous imprinted polymer and it reported minimum inhibitory concentration at 7.4 mg L-1. Within 10 min, reduced graphene oxide completely kills the E.coli while microporous imprinted polymer was embedded with the reduced graphene oxide takes about 13 min to disinfect the water. The reduced graphene oxide nanoparticles were near the imprinted cavity to generate localized temperature between 180 and 210 °C to kill the bacterial cells trapped inside the imprinted cavities of the polymer. The thermal atomic force microscope with the specialized heated probe tips were used to determine the localized temperature in the polymers. The localized thermal energy would be responsible for the production of superoxides, which were as similar to photolysis reactions, and would be further improving antibacterial activity. The combination of selective capture and destruction of pathogens in a single molecular construct improves disinfection of drinking water. Boron-containing mesoporous bioactive glass (B-MBG) scaffolds could be capable of promoting osteogenesis by activating Wnt/β-catenin signaling pathway during the process of bone defect repair. Despite this, more involving molecular controls are still largely unclear. In the present study, we identified that the downstream of Wnt/β-catenin signaling pathway named transcription factor 7-like 2 (TCF7L2) served as a key effector to promote boron-induced bone regeneration and osteogenesis through lipocalin 2 (LCN2). TCF7L2 was highly expressed in osteoblasts when treated with B-MBG scaffold extraction than MBG. LCN2, as a secreted bone factor, positively affected osteogenic differentiation of MC3T3-E1 and osteogenesis in vivo, which could be induced by TCF7L2. In addition, interference of TCF7L2 decreased the osteogenic differentiation of osteoblasts. Finally, we identified that rLCN2 could rescue the poor ability of osteogenic differentiation of MC3T3-E1 whose Tcf7l2 gene was knocked down by lentiviral transfection of shRNA. Our findings provide some new insights into the molecular controls of boron-associated bone regeneration and potential therapeutic targets for the treatment of bone defects. Periosteum as an important component in the construct of bone is mainly responsible for providing nourishment and regulating osteogenic differentiation. When bone defect happens, the functionality of periosteum will also be influenced, furthermore, it will finally hamper the process of bone regeneration. However, fabrication of an artificial periosteum with the capabilities in accelerating angiogenesis and osteogenesis in the defect area is still a challenge for researchers. In this study, we fabricated an organic-inorganic hybrid biomimetic periosteum by electrospinning, which can induce mineralization in situ and control the ions release for long-term in local area. Further, this system exhibited potential capabilities in promoting in vitro, which means the potentiality in accelerating bone regeneration in vivo. Calcium phosphate nanoparticles (CaPs) were fabricated by emulsion method, then CaPs were further incorporated with gelatin-methacryloyl (GelMA) by electrospinning fibers to construct the hybrid hydrogel fibers. The fibers exhibited satisfactory morphology and mechanical properties, additionally, controlled ions release could be observed for over 10 days. Further, significant mineralization was proved on the surface of hybrid fibers after 7 days and 14 days' co-incubation with simulated body fluid (SBF). Then, favorable biocompatibility of the hybrid fibers was approved by co-cultured with MC3T3-E1 cells. Finally, the hybrid fibers exhibited potential capabilities in promoting angiogenesis and osteogenesis by co-culture with HUVECs and MC3T3-E1 cells. This biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum provided a promising strategy to develop periosteum biomaterials with angiogenesis and osteogenesis capabilities. V.A series of Cu nanoparticles (NPs) have been prepared by a facile hydrothermal method at 180 °C using different concentrations of NaOH solutions and characterized by XRD, SEM, TEM and FT-IR spectra. Their antibacterial activities were assessed by means of Gram-positive S. aureus and Gram-negative E. coli bacteria, where various dosages (3, 5, 7, 10 mg) of the antibacterial agents were applied, and compared with that of the commercial CuSO4 salt. mTOR activation The antibacterial mechanism was explored based on series of control experiments. The results show that the NaOH concentration affects the crystallinity, crystal size and surface hydroxyl content of the Cu NPs, which significantly influence the antibacterial activities. Compared to the commercial CuSO4 salt, the four Cu samples prepared using no less then 4 mol L-1 of NaOH display excellent antibacterial activities with low concentrations of copper leachates, which is great beneficial to the practical applications. The experimental results support that the highly reactive and soluble copper species in the antibacterial system of the Cu NPs is a Cu (II)-peptide complex, but not free Cu2+ ions. The cellular response is the most crucial in vitro research. Materials' biocompatibility is determined based on cell proliferation and growth. Moreover, the topography of the scaffold surface is the key to enhance cell attachment and anchoring that importantly control further tissue development. Individual cell types have specific preferences regarding the type of surface and its geometry. In our research, we used poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) PHBV to produce two types of substrate a 3D structure of electrospun fibers and 2D flat films. The PHBV products were morphologically characterized by scanning electron microscopy (SEM). The cytocompatibility was evaluated with cell viability and proliferation using two different types of cells human osteoblast-like cells (MG-63) and NIH 3 T3 murine fibroblast cells. The behaviour of both cell types was compared on the similar PHBV fiber scaffolds and films using two types of polystyrene (PS) based substrate for the cell culture study unmodified PS that is not favourable for the attachment of cells and on tissue culture polystyrene (TCPS) plates, which are chemically modify to enhance cells attachment.mTOR activation